| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.224442 |
| Chromosome: | chromosome 2 |
| Location: | 943917 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079750 | HKR1,HIK,HIK1 | Histidine kinase response regulator of two-component system; (1 of 1) IPR000014//IPR001610//IPR001789//IPR003594//IPR003661//IPR004358//IPR005467//IPR011006 - PAS domain // PAC motif // Signal transduction response regulator, receiver domain // Histidine kinase-like ATPase, C-terminal domain // Signal transduction histidine kinase, dimerisation/phosphoacceptor domain // Signal transduction histidine kinase-related protein, C-terminal // Histidine kinase domain // CheY-like superfamily | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCATCAACACCACGTCGAATGAGTCCAG |
| Internal bar code: | GTAGCGCCGTGCCGGAGCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 98.9833 |
| LEAP-Seq n confirming: | 1363 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGAAAAGATGGGTGTGA |
| Suggested primer 2: | TACCACGCCCACATAGCATA |