Insertion junction: LMJ.RY0402.224453_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.224453
Chromosome:chromosome 2
Location:3241628
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTATGAACTGGCCGTCGCTGTACCGATGG
Internal bar code:TGACCTATGTTTATGCTTTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:315
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCAACAACACCAACAGC
Suggested primer 2:CGAATGTATGGGGGTATTGC