| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224473 |
| Chromosome: | chromosome 10 |
| Location: | 261671 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419500 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAATCGCGCGCCATACAATTGCAATTT |
| Internal bar code: | GTTAAGGACTGTACAACCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 97.8909 |
| LEAP-Seq n confirming: | 11557 |
| LEAP-Seq n nonconfirming: | 249 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATGACCGTGCGTGTATG |
| Suggested primer 2: | GCCTACTAAGCACACGCCTC |