| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224479 |
| Chromosome: | chromosome 1 |
| Location: | 3163679 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019750 | (1 of 1) PTHR10871:SF4 - DNA TOPOISOMERASE 6 SUBUNIT B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCGACACAGGTTGCCGGGCCTGCTGAT |
| Internal bar code: | TCGTACTTTACGTTCCAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 95.6266 |
| LEAP-Seq n confirming: | 12507 |
| LEAP-Seq n nonconfirming: | 572 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGTGGTAAGGCAG |
| Suggested primer 2: | GGGTGGTTCATTGCAGAACT |