| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224490 |
| Chromosome: | chromosome 11 |
| Location: | 3670867 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482483 | (1 of 1) K12599 - antiviral helicase SKI2 (SKI2, SKIV2L) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCCTTGCGCATGCAGTATGCATCGTGC |
| Internal bar code: | CAGATAAGAATACTAGGGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 99.5797 |
| LEAP-Seq n confirming: | 16110 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCAGAGGTGGAGCCTG |
| Suggested primer 2: | GTTTGGATAGCTGGGAGGGT |