| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224493 |
| Chromosome: | chromosome 12 |
| Location: | 7073074 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559555 | (1 of 4) 2.3.1.43 - Phosphatidylcholine--sterol O-acyltransferase / Phospholipid--cholesterol acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCACCCACTTGTCCTTCAGTCGTGCCTG |
| Internal bar code: | GGACGAACCGAGGCGCAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 39.0186 |
| LEAP-Seq n confirming: | 1320 |
| LEAP-Seq n nonconfirming: | 2063 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGCTCTCAAACACACAT |
| Suggested primer 2: | TACGACCTAGGCAGCGACTT |