Insertion junction: LMJ.RY0402.224506_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.224506
Chromosome:chromosome 10
Location:3016172
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g441050 (1 of 2) PTHR12321:SF55 - PHD FINGER PROTEIN ALFIN-LIKE 4 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCATCCTCACTGGTTGCTGTGCGCTGG
Internal bar code:GCATCCTGTCCGGCGCGGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:291
LEAP-Seq percent confirming:49.4028
LEAP-Seq n confirming:455
LEAP-Seq n nonconfirming:466
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTCGACGGACGGTTACTG
Suggested primer 2:TCGTCTACACAGCTCATCCG