| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224534 |
| Chromosome: | chromosome 10 |
| Location: | 991666 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424900 | PHX13,PFH1 | Prolyl 4-hydroxylase 1; (1 of 2) PTHR10869//PTHR10869:SF66 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // OXIDOREDUCTASE, 2OG-FE(II) OXYGENASE FAMILY PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTTTGTGAGCATGTGATCGGCGTACAG |
| Internal bar code: | CACCTTCTCCTACAGTCGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 95.539 |
| LEAP-Seq n confirming: | 514 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGCTGTGTGTGTGACAG |
| Suggested primer 2: | CCAAACGTTAGCATCCCACT |