Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.224541 |
Chromosome: | chromosome 3 |
Location: | 9141841 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g211969 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAATATTTCAGCACTGACGCTGTGCTT |
Internal bar code: | AATGGTCCCGGGATACGCGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 381 |
LEAP-Seq percent confirming: | 96.7238 |
LEAP-Seq n confirming: | 25272 |
LEAP-Seq n nonconfirming: | 856 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACCAAACCTGCACACACAC |
Suggested primer 2: | TACGAACAAGACCAGCAACG |