Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.224542 |
Chromosome: | chromosome 11 |
Location: | 340862 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTCCCTGGTCCGCAGTCCCGCTCTCTT |
Internal bar code: | GCCGGGGTGGTATAGTTTTTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 888 |
LEAP-Seq percent confirming: | 99.4088 |
LEAP-Seq n confirming: | 16815 |
LEAP-Seq n nonconfirming: | 100 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCATACACACGCACATACA |
Suggested primer 2: | GAATGAGCCCATAGCCGATA |