| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224546 |
| Chromosome: | chromosome 12 |
| Location: | 2999014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501000 | PPT1,TPT22,TPT20 | Putative UDP-GlcA transporter; (1 of 4) PTHR11132//PTHR11132:SF97 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGACCTGTGCTAATGGTCACACGCTTG |
| Internal bar code: | CTAGTGGCGAGGGTGAAAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 98.9577 |
| LEAP-Seq n confirming: | 9589 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTCCTTCTTTTTCGCAG |
| Suggested primer 2: | GAAGGTGAGAGGAGCGTGTC |