| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224554 |
| Chromosome: | chromosome 2 |
| Location: | 1421782 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083500 | FAO2 | (1 of 5) 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase / HNA-3-monooxygenase; FAD-dependent oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACGGGCAGTACGACTTCATGAGTAACA |
| Internal bar code: | CGTTAGGCGAATTTCCTACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.5791 |
| LEAP-Seq n confirming: | 8991 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGTCAGTTGCAGCCTTA |
| Suggested primer 2: | ATGGCACGTACACAGGATGA |