| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.224566 |
| Chromosome: | chromosome_8 |
| Location: | 1959776 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g367650 | MUT11 | Transcriptional repression protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTTGGCGCGCCCGTCCAGCGTGTTGCACA |
| Internal bar code: | CGGACAAAACGGGCAAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 98.8866 |
| LEAP-Seq n confirming: | 3908 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGGTACGAATGCGGGT |
| Suggested primer 2: | GGGATATGGGGAGGAGTTGT |