| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224574 |
| Chromosome: | chromosome 14 |
| Location: | 3866119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632783 | (1 of 1) K06634 - cyclin H (CCNH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCATCCTCCACCCCGTCACGCATGAAT |
| Internal bar code: | GCTAGGTTAAGCCACTTTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1091 |
| LEAP-Seq percent confirming: | 95.1906 |
| LEAP-Seq n confirming: | 3246 |
| LEAP-Seq n nonconfirming: | 164 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTTTTGATGTGGATGC |
| Suggested primer 2: | CCCTTTCAACTCTCACCTGC |