Insertion junction: LMJ.RY0402.224597_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.224597
Chromosome:chromosome 4
Location:2692026
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g223300 CCP1 (1 of 5) K15109 - solute carrier family 25 (mitochondrial carnitine/acylcarnitine transporter), member 20/29 (SLC25A20_29, CACT, CACL, CRC1); Low-CO2-inducible chloroplast envelope protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAGGTTTGGTTTCAGCAAAGGCCCTTG
Internal bar code:CAATACCCTCTGCTCCCTTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:98.9474
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACTGCTATTCTCCCTTC
Suggested primer 2:ACCTGCAGCTTGGACTTGAT