| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224619 |
| Chromosome: | chromosome 12 |
| Location: | 8438697 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548450 | GT90-24,GT90F24 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 24 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGGTCCAGAGGGGTACAGCGCGTGCGG |
| Internal bar code: | CGTGTGCCGCCTAGCGTCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 98.7069 |
| LEAP-Seq n confirming: | 1603 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGAGGCTCCTGACTGG |
| Suggested primer 2: | TGCTATGGCTGCAAGTTGAC |