| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224648 |
| Chromosome: | chromosome 3 |
| Location: | 1522348 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152100 | XRN2 | 5' to 3' exoribonuclease; (1 of 1) IPR004859//IPR027073//IPR029060 - Putative 5-3 exonuclease // 5'-3' exoribonuclease // PIN domain-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGCTGCAGCCCCGCCTCCGTGGCCGTC |
| Internal bar code: | GGCATTCATGGTCACATGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 99.5565 |
| LEAP-Seq n confirming: | 7632 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTGACGAGGAGGTGTA |
| Suggested primer 2: | AATGGGAGTGAGTGGAGGTG |