| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224725 |
| Chromosome: | chromosome 9 |
| Location: | 5211308 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399363 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCATCACCTCGGCATCCGCCACCGCCC |
| Internal bar code: | ATCATGGGGGTCCCGAACATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 98.2095 |
| LEAP-Seq n confirming: | 4059 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGGTTGGTTTAGGGCT |
| Suggested primer 2: | GCTCCTCCAGCACTGAAGTT |