| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224758 |
| Chromosome: | chromosome 7 |
| Location: | 647131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317100 | POB18 | Proteome of basal body 18 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGGGATTTGTGCACAACGAGGACTACG |
| Internal bar code: | AACCTGAGACGTCCCCGATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.7625 |
| LEAP-Seq n confirming: | 840 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGCAACGCCCTCATCTT |
| Suggested primer 2: | TTAACCCGTGTCTCACCTCC |