| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224781 |
| Chromosome: | chromosome 14 |
| Location: | 3182382 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629050 | (1 of 1) PF02298 - Plastocyanin-like domain (Cu_bind_like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCTGCCGTCCCGAGCGTCACGTCGGCC |
| Internal bar code: | TGCAATTCATTGAAATCATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGAAGGTCAGGGTCTC |
| Suggested primer 2: | GGTCTCGCTTTGCCTGATAG |