| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224794 |
| Chromosome: | chromosome 3 |
| Location: | 544893 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145387 | FAP239 | Coiled-Coil Flagellar Associated Protein 239 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACACCACTGCCCTAGAATTGCCATACA |
| Internal bar code: | TGCAGGACGATAATTAACACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 98.8568 |
| LEAP-Seq n confirming: | 1816 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTTATGCCTCCAAACAG |
| Suggested primer 2: | GACGTTACAGCAGACGTCCA |