Insertion junction: LMJ.RY0402.224833_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.224833
Chromosome:chromosome 10
Location:6333233
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g465350 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCGCGCCGCCATCTTGCCCGCTGCCGC
Internal bar code:ACCACTACCGGTTGCGCGTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:97.3684
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAATGCTCACCAAAGTGC
Suggested primer 2:AACCAACTTGTAGGCCATGC