| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224848 |
| Chromosome: | chromosome 12 |
| Location: | 1671628 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486000 | (1 of 102) PF01753 - MYND finger (zf-MYND) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAAAAGATCAAGCCTACCAGAGATTGGG |
| Internal bar code: | ATTAGTACTCGTAATTCCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 94.3511 |
| LEAP-Seq n confirming: | 618 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCTGGTCAAAGCAGATG |
| Suggested primer 2: | GCATTGTGTGAAGTTGGGTG |