| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224877 |
| Chromosome: | chromosome 9 |
| Location: | 3672612 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390652 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTTGGCAGGCCGGCAGCACCGCACCAC |
| Internal bar code: | ACGTAATGATCTATGGGTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 71.8327 |
| LEAP-Seq n confirming: | 584 |
| LEAP-Seq n nonconfirming: | 229 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGAGCTGGTAGGTGTG |
| Suggested primer 2: | TACCGCTGAAGCCACTTCTT |