| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224887 |
| Chromosome: | chromosome 16 |
| Location: | 6011013 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676450 | CPLD39 | Conserved in the Plant Lineage and Diatoms | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTACTGATGCCCCTTGCCCGATGTACG |
| Internal bar code: | ATGAAGGAACCAAAAGGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 99.652 |
| LEAP-Seq n confirming: | 859 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGGTAACGATTGCACTT |
| Suggested primer 2: | CAACGCCACCTCACCTTATT |