| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224909 |
| Chromosome: | chromosome 17 |
| Location: | 2571572 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716700 | (1 of 77) IPR029071 - Ubiquitin-related domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCACCCATGGACCCGTGTGGCTAACCC |
| Internal bar code: | TTGAGCGGAACACGGTGAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 97.2335 |
| LEAP-Seq n confirming: | 1195 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTAGTAGCGCGGTAGG |
| Suggested primer 2: | CTTTGCTCGAGGTTGAGACC |