| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224927 |
| Chromosome: | chromosome 12 |
| Location: | 5409077 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530000 | (1 of 11) 2.7.11.24 - Mitogen-activated protein kinase / Stress-activated protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAACTATGTGCGTGAGGTCACAGCGTT |
| Internal bar code: | ACCGTGGTTACTTAGTGAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 99.2735 |
| LEAP-Seq n confirming: | 2323 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGGGACACGGATGCTAC |
| Suggested primer 2: | GGTGGCATTTGTGGAGAAGT |