| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224928 |
| Chromosome: | chromosome 6 |
| Location: | 8700505 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309717 | (1 of 1) PTHR12735//PTHR12735:SF22//PTHR12735:SF3 - BOLA-LIKE PROTEIN-RELATED // SUBFAMILY NOT NAMED // PROTEIN T12D8.10-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATGCCTCCGCGGGGGTGCGGGTGACC |
| Internal bar code: | CATTTTTGTGCGGCCTTTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 99.5968 |
| LEAP-Seq n confirming: | 10870 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCCCACATTCTTGAGGT |
| Suggested primer 2: | TTCATGCAAGCAGTGGAAAG |