| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.224949 |
| Chromosome: | chromosome 13 |
| Location: | 2668043 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581801 | (1 of 1) PTHR13198//PTHR13198:SF4 - RING FINGER PROTEIN 25 // E3 UBIQUITIN-PROTEIN LIGASE RNF25 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCCTCCGCTGCGCTGCCATTCACGTGG |
| Internal bar code: | GCTGGCATCGGGCAGGGTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 98.5185 |
| LEAP-Seq n confirming: | 266 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTTGCGCATTAGAGTG |
| Suggested primer 2: | CACTACATTCGCAGAGGCAG |