Insertion junction: LMJ.RY0402.224989_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.224989
Chromosome:chromosome 9
Location:6771790
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g409350 RLI1 (1 of 1) K06174 - ATP-binding cassette, sub-family E, member 1 (ABCE1); Fe-S ABC transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACGCCAAACCCACACCACCCCGGCGC
Internal bar code:CACCCCGCTATTTGTAACCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:518
LEAP-Seq percent confirming:94.0
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGTCTTACATTGGTCGTT
Suggested primer 2:ACCCGTACTCCTCCACTCCT