Insertion junction: LMJ.RY0402.225413_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.225413
Chromosome:chromosome 7
Location:2975995
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g333050 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCATGCGCACCCGAAAGGCGAAAGTCCA
Internal bar code:GTCTAACGAGTTGCGGGGTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:575
LEAP-Seq percent confirming:99.1226
LEAP-Seq n confirming:4519
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGTCACAGGCACCATAC
Suggested primer 2:CTGCTGCTAGTCCATCCACA