| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225422 |
| Chromosome: | chromosome_6 |
| Location: | 7276907 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g298500 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCTCCCTCCCGCCTGCCACTGCACAGGCC |
| Internal bar code: | GCGATTGCAGTGGCCAAATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 90.4159 |
| LEAP-Seq n confirming: | 500 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCTGTGTACACCTGCC |
| Suggested primer 2: | CTGACACGCTAAGCAAACCA |