| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225429 |
| Chromosome: | chromosome 16 |
| Location: | 1815348 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655400 | (1 of 1) K17985 - activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATAGGGAGCGCAGGCGGCAGGCAGCAGG |
| Internal bar code: | TTGTTACCTTTCGGTTGGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 85.7277 |
| LEAP-Seq n confirming: | 913 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGATGGTGTGTGAAGC |
| Suggested primer 2: | CATTAGCAACGAGCACCTGA |