| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225441 |
| Chromosome: | chromosome 7 |
| Location: | 4126740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342800 | CGL16 | (1 of 1) PF07063 - Domain of unknown function (DUF1338) (DUF1338); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCAAGCAGCCGGGGACTCCGGGCACGCA |
| Internal bar code: | GAGGAGGCGCGGCCTTATGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTCAGTGAGTAGCCGTG |
| Suggested primer 2: | TTCCCAACAAATCCCTTACG |