| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225442 |
| Chromosome: | chromosome 12 |
| Location: | 7222467 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558350 | (1 of 1) PF02609 - Exonuclease VII small subunit (Exonuc_VII_S) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAACCCTGCCTGTGTGTCAAGAGCTTTC |
| Internal bar code: | CGCCGATCGAATAACGGATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.6255 |
| LEAP-Seq n confirming: | 798 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGCTGTCTGCTTACGG |
| Suggested primer 2: | ATCACCGACCTACCTTGCAC |