| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.225442 |
| Chromosome: | chromosome 12 |
| Location: | 7222471 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558350 | (1 of 1) PF02609 - Exonuclease VII small subunit (Exonuc_VII_S) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGTTGGACCGGTGGACTAAGTGGACGT |
| Internal bar code: | TAGAGAGGGGACTGCTAGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.7067 |
| LEAP-Seq n confirming: | 3399 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGCTGTCTGCTTACGG |
| Suggested primer 2: | ATCACCGACCTACCTTGCAC |