| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.225477 |
| Chromosome: | chromosome 14 |
| Location: | 216225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGGATGGATAAGTGGGGGCGTGGGCTT |
| Internal bar code: | ACTGTCACTGTTTGTAACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 94.7235 |
| LEAP-Seq n confirming: | 2998 |
| LEAP-Seq n nonconfirming: | 167 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGTAACCACCACCCCAC |
| Suggested primer 2: | CTCGGTCATTTGGAAGGTGT |