Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.225492 |
Chromosome: | chromosome_16 |
Location: | 7496140 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g687406 | antisense | intron|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTCCAGGACACTGGCTCTCGGTGTTACGC |
Internal bar code: | GCGGTCCACGTATCACGCGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 744 |
LEAP-Seq percent confirming: | 98.6622 |
LEAP-Seq n confirming: | 3540 |
LEAP-Seq n nonconfirming: | 48 |
LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAACAACCCTCCCTGACAGA |
Suggested primer 2: | CGTTGACGTGGTTGTAATCG |