| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225492 |
| Chromosome: | chromosome 16 |
| Location: | 7496140 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687406 | POB20 | Proteome of basal body 20 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCAGGACACTGGCTCTCGGTGTTACGC |
| Internal bar code: | GCGGTCCACGTATCACGCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 98.6622 |
| LEAP-Seq n confirming: | 3540 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAACCCTCCCTGACAGA |
| Suggested primer 2: | CGTTGACGTGGTTGTAATCG |