| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.225509 |
| Chromosome: | chromosome 16 |
| Location: | 6101404 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675850 | (1 of 1) K11729 - acyl-CoA dehydrogenase family member 10 (ACAD10) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGGGGGTTGTAACTGCCAGCCTAAAAT |
| Internal bar code: | TTACCCCTGAAAGATCGCTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 98.9241 |
| LEAP-Seq n confirming: | 4965 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGCTACTGCTGCTACTG |
| Suggested primer 2: | CGTGTGCCTGCTCTGTGTAT |