Insertion junction: LMJ.RY0402.225538_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.225538
Chromosome:chromosome 16
Location:1427933
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g652400 FAL18,FAP183 (1 of 17) PF13202 - EF hand (EF-hand_5); EF-hand domain Flagellar Associated Protein 183 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTCACCCAGTTGGCACGCAGGTGGTACT
Internal bar code:TGAGGTCTAGCGATCGCTTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:478
LEAP-Seq percent confirming:99.5283
LEAP-Seq n confirming:211
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTGGATGTTGTTGTCGG
Suggested primer 2:AGCCTGGTTCAGTATGTGGG