| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.225546 |
| Chromosome: | chromosome 15 |
| Location: | 226419 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635400 | ZYS3,ZYS3-1 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Zygote-specific protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTTTGGCATCGTGCGCATTTGTTGCGT |
| Internal bar code: | AGTCTGTCTTGGGTCGGCAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 98.9682 |
| LEAP-Seq n confirming: | 16786 |
| LEAP-Seq n nonconfirming: | 175 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACACACCTACACAGCC |
| Suggested primer 2: | CTACCAGTCAGGGGTGCATT |