| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.225566 |
| Chromosome: | chromosome 3 |
| Location: | 4183877 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173850 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAGCAATACCCCCTGTGCCCTCAGCTT |
| Internal bar code: | CACTCGGGTAAAGCGGCACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 99.0244 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCGTGTACGGGTATCTG |
| Suggested primer 2: | TGCATGTGTTCAATGTGTGG |