| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.225577 |
| Chromosome: | chromosome 2 |
| Location: | 4763965 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102551 | CSV1 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGCCAGAGTCCGGCCCGTGAAGGAGGC |
| Internal bar code: | ACCGGGTTAGTTCGCATGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 80.2099 |
| LEAP-Seq n confirming: | 535 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCACAGTGATCTGCAGCG |
| Suggested primer 2: | CCGGTACTACGGTCTCCAAA |