| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.225589 |
| Chromosome: | chromosome 7 |
| Location: | 266324 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314000 | (1 of 1) PF16156 - Domain of unknown function (DUF4864) (DUF4864) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTGTAGCTGTTGGCGGACAGCATCTT |
| Internal bar code: | GACAGTGGTCTGAGTCAAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.6594 |
| LEAP-Seq n confirming: | 6437 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACACACGAGGAATGG |
| Suggested primer 2: | ACCCATGTAACCTGAGGCTG |