| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225623 |
| Chromosome: | chromosome 16 |
| Location: | 3911729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690500 | NHP2 | Nucleolar protein, Small subunit of H/ACA snoRNPs; (1 of 1) PTHR23105//PTHR23105:SF61 - RIBOSOMAL PROTEIN L7AE FAMILY MEMBER // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGTCACGTGTCAAGTCAGTGGTCCTAA |
| Internal bar code: | GAGGGGGGGCGAAGGAGAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 98.7903 |
| LEAP-Seq n confirming: | 1960 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGCGGAGAGCTGGAGAC |
| Suggested primer 2: | ACCGAGGTGGAGAAGAAGGT |