| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225641 |
| Chromosome: | chromosome 11 |
| Location: | 2969832 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478184 | (1 of 1) 3.2.1.25 - Beta-mannosidase / Mannase | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGTTGGTAGATCCACGGCTCGAACAG |
| Internal bar code: | TTTCTTAGCTGGCGGCGGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 97.9834 |
| LEAP-Seq n confirming: | 826 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTAGAAACCAGCCCAA |
| Suggested primer 2: | TGGCTACGCCTTCACTTCTT |