| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225677 |
| Chromosome: | chromosome 12 |
| Location: | 2115052 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509100 | FAP307 | Flagellar Associated Protein 307 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAGCGGGTCTCACGGATGCCGAACTGG |
| Internal bar code: | AAGAGAGGAGTCGACCGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 98.3985 |
| LEAP-Seq n confirming: | 1536 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCACACCTACGTCCTAAT |
| Suggested primer 2: | CGTCACTCCTGACCCTCAAT |