Insertion junction: LMJ.RY0402.225702_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.225702
Chromosome:chromosome 3
Location:7283531
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g207150 (1 of 1) 1.13.12.3 - Tryptophan 2-monooxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGTGGGGGCAATGCACATTGAAACGTA
Internal bar code:TCGGATCGGCTACTAATCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:828
LEAP-Seq percent confirming:99.1336
LEAP-Seq n confirming:4920
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGAAGGAGAGTTACGGGT
Suggested primer 2:CCACCAGCTTGTGTACCCTT