Insertion junction: LMJ.RY0402.225702_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.225702
Chromosome:chromosome 3
Location:7283554
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g207150 (1 of 1) 1.13.12.3 - Tryptophan 2-monooxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGCCTGCAGGCGCCTTCATCCAGGGCAG
Internal bar code:GCCAATCCGTGTATCCGGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:611
LEAP-Seq percent confirming:97.167
LEAP-Seq n confirming:1612
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGAAGGAGAGTTACGGGT
Suggested primer 2:CCACCAGCTTGTGTACCCTT