| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225707 |
| Chromosome: | chromosome 1 |
| Location: | 4375562 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029600 | (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGATAGTGCGGCACTACCAGGGCAGTAC |
| Internal bar code: | GAGAGTCACGCTCGGTCATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 98.4186 |
| LEAP-Seq n confirming: | 1058 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCACTTCGTTCTCCACA |
| Suggested primer 2: | CCTCTGAGCTCAACCTGACC |